SECTION: Physics, Nanotechnologies, Materials Technology, Space
SCIENTIFIC ORGANIZATION:
Laboratory of Nano-Bioengineering, National Research Nuclear University (Moscow Engineering Physics Institute), Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Department of Biophysics, Lomonosov Moscow State University.
REPORT FORM:
«Poster report»
AUTHOR(S)
OF THE REPORT:
Solovyeva D.O., Chistyakov, A.A., Lukashev E.P., Mochalov K.E., Oleinikov V.A., Nabiev I.R.
SPEAKER:
Daria Solovyeva
REPORT TITLE:
Silver nanoparticles strongly affect the properties of Halobacterium salinarum photosensitive purple membranes containing the membrane protein bacteriorhodopsin.
TALKING POINTS:

The processes of silver nanoparticles (AgNPs) interactions with purple membranes (PM) containing bacteriorhodopsin (BRh) were studied by the absorption spectroscopy, atomic force microscopy, Raman and SERS spectroscopy. The presence of PM suppresses the forming of AgNPs aggregates which are necessary for SERS. It determines low value of SERS enhancement factor. It was shown that enhancement of Raman signal has electromagnetic nature. Comparison of the optical spectroscopy and atomic force microscopy data allows to suppose that SERS-spectra arise from the BRh localized in so called “hot spots”, which are formed by AgNPs aggregation caused by incubation with SERS-activator. SERS-spectroscopy shows, that Ag NPs modify the BRh structure localized in the “hot spots”. Their structures close to dark-adapted BR548 form, contains 13-cis retinal with cis-C=N fragment. It was found that this type of Ag NPs impact suppresses the BRh’s photocycle.

This study was supported by the Ministry of Education and Science of the Russian Federation, grant no. 11.G34.31.0050, by the European Community through the ERA-Net ICENAP project, and by the RFBR no. 13-04-00168.